Faculty Collaboration Database

Effects of human breast stromal cells on conjugated linoleic acid (CLA) modulated vascular endothelial growth factor-A (VEGF-A) expression in MCF-7 cells. Anticancer Res 2005;25(6B):4061-8

Date

11/30/2005

Pubmed ID

16312046

Scopus ID

2-s2.0-27944501632 (requires institutional sign-in at Scopus site)   16 Citations

Abstract

BACKGROUND: Conjugated linoleic acid (CLA), a naturally occurring compound found in ruminant dairy and beef products, has been shown to possess anti-cancer ability in vivo and in vitro. There are several CLA isomers in ruminant-produced food products, among which t10,c12-CLA and c9,t11-CLA are most potent. Vascular endothelial growth factor-A (VEGF-A) has been implicated as an angiogenesis-activating cytokine. Our previous results indicated that CLA induced suppression of VEGF-A in MCF-7 cells, which may be one of CLA's anticancer mechanisms.

MATERIALS AND METHODS: The effects of 10,c12-CLA and c9,t11-CLA on VEGF-A mRNA and protein expression in MCF-7 cells, which were co-cultured with human breast stromal cells isolated from breast tissues of surgical specimens of mammoplasty and breast cancer patients, were detected by RT-PCR and Western blot analysis.

RESULTS: VEGF-A mRNA and protein expressions were significantly (p < 0.05) elevated in co-cultured MCF-7 cells in comparison with cultured MCF-7 cells alone. Normal human breast stromal cells contribute greater effects in increasing VEGF-A protein expression in MCF-7 cells. Both t10,c12-CLA and c9,t11-CLA significantly (p < 0.05) decreased VEGF-A mRNA and protein levels in co-cultured MCF-7 cells. t10,c12-CLA appeared to be the more active isomer than c9, t11-CLA.

CONCLUSION: The results indicate that dietary CLA might serve as a chemo-therapeutic agent in human breast cancers by down-regulating VEGF-A expression.

Author List

Wang LS, Huang YW, Sugimoto Y, Liu S, Chang HL, Ye W, Shu S, Lin YC

MESH terms used to index this publication - Major topics in bold

Biomarkers, Tumor
Breast Neoplasms
Cell Communication
Cell Line, Tumor
Coculture Techniques
Down-Regulation
Humans
Isomerism
Linoleic Acids, Conjugated
Neovascularization, Pathologic
RNA, Messenger
Stromal Cells
Vascular Endothelial Growth Factor A