Growth of AKR T cell leukemia lymphoblasts in medium containing interleukin 2 (IL-2). In Vitro 1983 Oct;19(10):759-66
Date
10/01/1983Pubmed ID
6605296DOI
10.1007/BF02618095Scopus ID
2-s2.0-0020980058 (requires institutional sign-in at Scopus site) 9 CitationsAbstract
Long-term cloned mouse leukemic T cell lines were established in vitro using interleukin-2 (IL-2) conditioned media. The cell lines were tested for retention of both antigenic expression and tumorigenicity, as well as for growth characteristics. Several important findings resulted from these studies. A reliable method was developed for consistent success in the culturing and cloning of leukemic T cell lines. Cultured cells from IL-2-dependent lines were found to retain their original histocompatibility and differentiation antigen characteristics for up to 2 yr. Mortality patterns, comparing long-term cloned leukemic T cell lines with fresh AKR leukemia cells, showed that the cloned cells had greater tumorigenicity. An especially interesting finding was that cell lines established both from different mice and from a single organ of an individual mouse were heterogeneous with respect to antigenic makeup, cell growth kinetics, and tumorigenicity. Finally, because of their dependence on IL-2, the cloned tumor cell lines provided excellent index cells to quantify IL-2 activity.
Author List
Shih CY, Truitt RL, Andreani M, Bortin MMMESH terms used to index this publication - Major topics in bold
AnimalsAntigens, Surface
Cell Division
Cell Line
Clone Cells
Culture Media
Histocompatibility Antigens
Interleukin-2
Kinetics
Leukemia, Lymphoid
Mice
Mice, Inbred AKR
T-Lymphocytes
