Faculty Collaboration Database

Use of monoclonal antibody to increase sensitivity and specificity in quantitative immunodiffusion assays. J Immunol Methods 1982;48(1):13-22

Date

01/01/1982

Pubmed ID

6799577

DOI

10.1016/0022-1759(82)90205-8

Scopus ID

2-s2.0-0020036467 (requires institutional sign-in at Scopus site)   5 Citations

Abstract

A mouse monoclonal antibody has been produced which recognizes human liver gamma-cystathionase, Radioiodination of the monoclonal antibody facilitated its use in combination with non-specific precipitating rabbit antisera in classical immunodiffusion assays. The technique may have broad applicability in the detection and quantitation of rare antigens which are difficult to purify but easily recognizable in immunodiffusion assays. It may also be used for the initial detection of monoclonal antibodies to unique antigens of interest.

Author List

Glode LM, Montgomery RR, Smith CG, Link DB

MESH terms used to index this publication - Major topics in bold

Animals
Antibodies, Monoclonal
Antibody Specificity
Antigen-Antibody Complex
Cystathionine gamma-Lyase
Humans
Immune Sera
Immunodiffusion
Immunoelectrophoresis, Two-Dimensional
Liver
Precipitins
Rabbits