BK virus (BKV): infection, propagation, quantitation, purification, labeling, and analysis of cell entry. Curr Protoc Cell Biol 2009 Mar;Chapter 26:Unit 26.2
Date
03/14/2009Pubmed ID
19283732Pubmed Central ID
PMC2818100DOI
10.1002/0471143030.cb2602s42Scopus ID
2-s2.0-63649105302 (requires institutional sign-in at Scopus site) 18 CitationsAbstract
BK virus (BKV) can cause BKV nephritis in renal transplant patients and has become a significant reason for graft loss in this decade. BKV is latent in the urogenital tract and most likely is transported with the donor kidney to recipients. BKV replication occurs in the nucleus of human renal proximal tubular cells (HRPTEC) and daughter viruses are delivered to other cells to spread infection. A few in vitro studies have been reported about the mechanism and kinetics of BKV infection. However, there are still a lot of unknown factors regarding BKV infection. This unit describes the handling of BKV, BKV propagation, determination of titer and ability to infect cells, as well as purification and labeling of BKV in order to analyze BKV cell entry.
Author List
Moriyama T, Sorokin AAuthor
Andrey Sorokin PhD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
BK VirusCell Culture Techniques
Epithelial Cells
Fluorescent Dyes
Humans
Kidney Tubules, Proximal
Microscopy, Confocal
Polyomavirus Infections
Protein Transport
Tumor Virus Infections
Viral Proteins
Virology
Virus Internalization
Virus Replication