Rod photoreceptor disc shedding in eye cups: relationship to bicarbonate and amino acids. Exp Eye Res 1983 Apr;36(4):567-79
Date
04/01/1983Pubmed ID
6852133DOI
10.1016/0014-4835(83)90051-9Scopus ID
2-s2.0-0020581440 (requires institutional sign-in at Scopus site) 44 CitationsAbstract
We previously demonstrated that disc shedding in eye cups from Xenopus laevis depends on medium bicarbonate concentration, but also found that other unidentified medium components influenced the response. Here, in order to determine which medium components influence shedding, we used high bicarbonate salt solutions supplemented with medium components in amphibian tissue culture medium. Compared to the high bicarbonate salt solution alone, addition of fetal bovine serum or whole egg ultrafiltrate enhanced disc shedding. However, a response fully comparable to that in intact animals was obtained on addition of a mixture of 14 amino acids. The full light-evoked response depended on the simultaneous presence of high bicarbonate (35 mM) and amino acids. In the presence of amino acids, photoreceptor tips and RPE showed tight interdigitation and less tendency for in vitro separation, suggesting that these medium components promote such interdigitation. Using the defined medium we have additionally shown that shedding is related to HCO3- concentration under conditions of controlled medium pH, Cl- content and osmolality.
Author List
Besharse JC, Dunis DAAuthor
Joseph C. Besharse PhD, MA Emeritus Professor in the Cell Biology Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Amino AcidsAnimals
Bicarbonates
Culture Media
Culture Techniques
Isotonic Solutions
Light
Phagocytosis
Photoreceptor Cells
Pigment Epithelium of Eye
Rod Cell Outer Segment
Xenopus laevis
