The paracellular permeability of opossum kidney cells, a proximal tubule cell line. Kidney Int 1999 Dec;56(6):2304-8
Date
12/14/1999Pubmed ID
10594810DOI
10.1046/j.1523-1755.1999.00787.xScopus ID
2-s2.0-0033405975 (requires institutional sign-in at Scopus site) 18 CitationsAbstract
UNLABELLED: The paracellular permeability of opossum kidney cells, a proximal tubule cell line.
BACKGROUND: The regulation of the unusually leaky paracellular pathway of the proximal tubule is poorly understood partially because of the lack of an appropriate in vitro cell model. In this study, we determined whether the paracellular permeability of opossum kidney (OK) cells would resemble that of the in vivo proximal tubule epithelium.
METHODS: The parental and subclonal OK cells and, for comparison, LLC-PK1 cells were cultured on permeable Transwell supports. The apparent paracellular permeability coefficient (Papp) for the extracellular marker 3H-mannitol was determined.
RESULTS: The Papp of OK cell sheets (12.17 x10-6 cm/sec) was remarkably close to the previously reported Papp of rat proximal tubules. The Papp of LLC-PK1 cells, another proximal tubule cell line, however, was approximately 20-fold lower than that of both OK cells and the in vivo proximal tubule. Phorbol 12-myristate 13-acetate, a protein kinase C activator, enhanced the Papp of OK cell sheets. The characteristic response of paracellular permeability to Ca2+ switch was demonstrated in OK cell sheets. Slight variations of Papp among several OK subclones were observed. Basal to apical Papp was uniformly higher than apical to basal Papp, independent of cell subtype. This rectification was attenuated by inhibition of active transport.
CONCLUSIONS: OK cell sheets cultured on Transwell supports possess a leaky paracellular pathway resembling that of the proximal tubule epithelium in vivo.
Author List
Liang M, Ramsey CR, Knox FGMESH terms used to index this publication - Major topics in bold
AmilorideAnimals
Anti-Arrhythmia Agents
Calcium
Carcinogens
Cell Culture Techniques
Cell Membrane Permeability
Clone Cells
Kidney Tubules, Proximal
LLC-PK1 Cells
Mannitol
Nephrons
Opossums
Sodium
Swine
Tetradecanoylphorbol Acetate
Tight Junctions
Tritium
Urothelium
