Diversity and modulation of plasminogen activator activity in human prostate carcinoma cell lines. Prostate 1995 Oct;27(4):179-86
Date
10/01/1995Pubmed ID
7479384DOI
10.1002/pros.2990270402Scopus ID
2-s2.0-0028802450 (requires institutional sign-in at Scopus site) 29 CitationsAbstract
Baseline cellular plasminogen activator (PA) activity, and the cellular proteins responsible for variations in PA activity were evaluated in three human prostate carcinoma cell lines. Net PA activity in the cell lines PC-3, DU-145, and LNCaP was measured using a plasminogen-dependent fibrin lysis assay. These three cell lines were then analyzed to determine the specific protein(s) responsible for differences in PA activity. mRNA and protein levels of cellular urinary PA (uPA), tissue PA (tPA), PA inhibitor 1 (PAI1), PA inhibitor 2 (PAI2), and uPA receptor (uPAr) were measured using Northern analysis and ELISA assays. Net cellular PA activity in the three cell lines varied over a 3-fold range (PC3 > DU145 >> LNCaP). Net PA activity in the fibrinolysis assay demonstrated a direct correlation with mRNA transcript levels of uPA, tPA, PAI1, and uPAr (PC-3 > DU-145 > LNCaP). uPA protein was identified in both the PC-3 and the DU-145 lines. tPA, PAI1, and PAI2 proteins were identified only in PC-3 cells. In general, cellular protein levels correlated with mRNA levels. These findings demonstrate that prostate carcinoma cell lines vary in their net PA activity. This variability results from both qualitative and quantitative differences in the cellular expression of PA regulatory proteins.
Author List
Lyon PB, See WA, Xu Y, Cohen MBMESH terms used to index this publication - Major topics in bold
Blotting, NorthernEnzyme-Linked Immunosorbent Assay
Humans
Male
Plasminogen Activator Inhibitor 1
Plasminogen Activator Inhibitor 2
Plasminogen Activators
Prostatic Neoplasms
RNA, Messenger
Receptors, Cell Surface
Receptors, Urokinase Plasminogen Activator
Tissue Plasminogen Activator
Tumor Cells, Cultured
Urokinase-Type Plasminogen Activator
