Zebrafish genetic map with 2000 microsatellite markers. Genomics 1999 Jun 15;58(3):219-32
Date
06/22/1999Pubmed ID
10373319DOI
10.1006/geno.1999.5824Scopus ID
2-s2.0-0033564291 (requires institutional sign-in at Scopus site) 304 CitationsAbstract
The zebrafish is the first vertebrate organism used for large-scale genetic screens seeking genes critical to development. These screens have been quite successful, with more than 1800 recessive mutations discovered that speak to morphogenesis of the vertebrate embryo. The cloning of the mutant genes depends on a dense genetic map. The 2000 markers we present here, using microsatellite (CA) repeats, provides 1.2-cM average resolution. One centimorgan in zebrafish is about 0. 74 megabase, so, for many mutations, these markers are close enough to begin positional cloning by YAC walks.
Author List
Shimoda N, Knapik EW, Ziniti J, Sim C, Yamada E, Kaplan S, Jackson D, de Sauvage F, Jacob H, Fishman MCMESH terms used to index this publication - Major topics in bold
AnimalsCloning, Molecular
Female
Gene Library
Genetic Linkage
Genome
Male
Microsatellite Repeats
Mutation
Physical Chromosome Mapping
Zebrafish
