Faculty Collaboration Database

Different roles for KIF17 and kinesin II in photoreceptor development and maintenance. Dev Dyn 2009 Sep;238(9):2211-22

Date

04/23/2009

Pubmed ID

19384852

Pubmed Central ID

PMC2733920

DOI

10.1002/dvdy.21956

Scopus ID

2-s2.0-70349097740 (requires institutional sign-in at Scopus site)   47 Citations

Abstract

Kinesin 2 family members are involved in transport along ciliary microtubules. In Caenorhabditis elegans channel cilia, kinesin II and OSM-3 cooperate along microtubule doublets of the axoneme middle segment, whereas OSM-3 alone works on microtubule singlets to elongate the distal segment. Among sensory cilia, vertebrate photoreceptors share a similar axonemal structure with C. elegans channel cilia, and deficiency in either kinesin II or KIF17, the homologue of OSM-3, results in disruption of photoreceptor organization. However, direct comparison of the two effects is confounded by the use of different species and knockdown strategies in prior studies. Here, we directly compare the effects of dominant-negative kinesin II and KIF17 expression in zebrafish cone photoreceptors. Our data indicate that dominant-negative kinesin II disrupts function at the level of the inner segment and synaptic terminal and results in cell death. In contrast, dominant-negative KIF17 has no obvious effect on inner segment or synaptic organization but has an immediate impact on outer segment assembly.

Author List

Insinna C, Humby M, Sedmak T, Wolfrum U, Besharse JC

MESH terms used to index this publication - Major topics in bold

Animals
Blotting, Western
Embryo, Nonmammalian
Immunohistochemistry
Immunoprecipitation
Mice
Microscopy, Electron, Transmission
Microscopy, Immunoelectron
Retinal Cone Photoreceptor Cells
Zebrafish
Zebrafish Proteins