Faculty Collaboration Database

Correlation of radiobiological assays of hypoxic fraction with phosphorus-31 magnetic resonance spectroscopy across multiple tumor lines. Radiat Res 1995 Jul;143(1):45-53

Date

07/01/1995

Pubmed ID

7597143

DOI

10.2307/3578924

Scopus ID

2-s2.0-0029035003 (requires institutional sign-in at Scopus site)   10 Citations

Abstract

Hypoxic fractions were measured in multiple tumor models and compared to 31P magnetic resonance spectroscopy (MRS) parameters. Hypoxic fractions were measured by excision assays in intramuscular and subcutaneous RIF-1 tumors, large and small SCCVII tumors, and KHT tumors. The hypoxic fractions ranged from 3% (small SCCVII) to 21% (KHT). Measurements of 31P MRS parameters (indices of tumor metabolism) were made under the same conditions used in the hypoxic fraction assays. The PCR/Pi ratios in the five tumor models were strongly correlated (r2 = 0.98, tau = 1.00, P < 0.02) with their hypoxic fractions, and the NTP/Pi ratio was weakly correlated (r2 = 0.67, tau = 0.60, P < 0.17) with hypoxic fraction. Smaller values of the PCr/Pi and NTP/Pi ratios were found in the tumors with larger hypoxic fractions. These experiments indicate that 31P MRS may provide a relative measurement of hypoxic fractions across different tumor lines that can be applied non-invasively to individual tumors.

Author List

Li SJ, Jin GY, Fish BL, Moulder JE

Author

Shi-Jiang Li PhD, MS Emeritus Professor in the Biophysics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Cell Hypoxia
Magnetic Resonance Spectroscopy
Mice
Mice, Inbred C3H
Neoplasms, Experimental
Oxygen
Phosphorus Isotopes
Polarography
Radiobiology
Tumor Cells, Cultured