Faculty Collaboration Database

Cytosolic phospholipase A2 gene in human and rat: chromosomal localization and polymorphic markers. Genomics 1995 Mar 01;26(1):138-41

Date

03/01/1995

Pubmed ID

7782073

DOI

10.1016/0888-7543(95)80093-2

Scopus ID

2-s2.0-0028987885 (requires institutional sign-in at Scopus site)   82 Citations

Abstract

We report the chromosomal localization and a simple sequence repeat (SSR) in the cytosolic phospholipase A2 (cPLA2) gene in both human and rat. A (CA)18 repeat in the promoter of the rat gene was determined to exhibit length polymorphism when analyzed using the polymerase chain reaction (PCR) in 19 different inbred rat strains. Genotyping for this marker in 234 F2 progeny of a SHR x BN intercross mapped the gene to rat chromosome 13. Using a PCR strategy, a fragment of the promoter for the human gene was isolated, and a (CA)18 repeat was identified. Since this marker displayed a low heterozygosity index, we also identified a mononucleotide repeat in the promoter for cPLA2 that displayed a polymorphism information content value of 0.76. The human gene was mapped using fluorescence in situ hybridization (FISH) to chromosome 1q25. Of interest, the gene encoding the enzyme prostaglandin-endoperoxide synthase 2 (cyclooxygenase-2), which acts on the arachidonic acid product of cPLA2, was previously localized to this same chromosomal region, raising the possibility of coordinate regulation. Identification of intragenic markers may facilitate studies of polymorphic variants of these genes as candidates for disorders in which perturbations of the eicosanoid cascade may play a role.

Author List

Tay A, Simon JS, Squire J, Hamel K, Jacob HJ, Skorecki K

MESH terms used to index this publication - Major topics in bold

Animals
Base Sequence
Chromosome Mapping
Chromosomes, Human, Pair 1
Crosses, Genetic
Female
Humans
In Situ Hybridization, Fluorescence
Male
Molecular Sequence Data
Phospholipases A
Phospholipases A2
Polymerase Chain Reaction
Polymorphism, Genetic
Promoter Regions, Genetic
Rats
Rats, Inbred Strains
Repetitive Sequences, Nucleic Acid