Mucin gene 19 (MUC19) expression and response to inflammatory cytokines in middle ear epithelium. Glycoconj J 2009 Dec;26(9):1275-84
Date
06/18/2009Pubmed ID
19533339Pubmed Central ID
PMC2918236DOI
10.1007/s10719-009-9245-xScopus ID
2-s2.0-77649266739 (requires institutional sign-in at Scopus site) 32 CitationsAbstract
Mucin gene 19 (MUC19) has been identified as a major gel-forming mucin in the human middle ear (ME). The objectives of this investigation were to characterize the expression and assess the regulation of MUC19 in the ME cell culture models utilized in the study of otitis media (OM). Findings demonstrate that MUC19 is expressed in both human immortalized cell culture (HMEEC) and chinchilla primary epithelial culture (CMEEC). ME exposure to inflammatory cytokines TNF-alpha, IL-1beta, IL-6 and IL-8 up-regulate MUC19 transcription, most robustly after exposure to TNF-alpha. Kinetic experiments suggest a relative early response in MUC19 transcription and a down-regulation after prolonged exposure. Glycoprotein production was increased in response to the increased transcription as well. Similar to other mucin genes in the ME, MUC19 is differentially regulated after exposure to inflammatory cytokines. The large size, gel-forming properties and up-regulation in response to important inflammatory cytokines of MUC19 suggest that it has significant potential to play a role in both physiology and pathophysiology of the ME.
Author List
Kerschner JE, Khampang P, Erbe CB, Kolker A, Cioffi JAMESH terms used to index this publication - Major topics in bold
AnimalsChinchilla
Cytokines
Ear, Middle
Epithelial Cells
Epithelium
Gene Expression Regulation
Humans
Inflammation Mediators
Interleukin-1beta
Interleukin-6
Interleukin-8
Mucins
Polymerase Chain Reaction
Time Factors
Tumor Necrosis Factor-alpha
