Faculty Collaboration Database

Elevated follicular fluid angiotensin II and pregnancy outcome. Fertil Steril 1995 Mar;63(3):528-34

Date

03/01/1995

Pubmed ID

7851582

DOI

10.1016/s0015-0282(16)57421-4

Scopus ID

2-s2.0-0028944911 (requires institutional sign-in at Scopus site)   15 Citations

Abstract

OBJECTIVE: To assess whether a relationship exists between follicular fluid (FF) angiotensin II (AII) concentration and pregnancy outcome or earlier fecundity parameters and whether correlations exist among FF AII concentrations and P, E2, T, androstenedione (A), or various ratios of these.

DESIGN: Retrospective study in which hormone concentrations in FF samples were measured.

SETTING: In vitro fertilization clinic-Assisted Reproductive Technology Program, Rush Medical Center.

PATIENTS: Twenty-six female patients underwent ovarian stimulation for IVF.

INTERVENTION: Leuprolide acetate was combined with hMG and FSH for ovarian stimulation.

MAIN OUTCOME MEASURE: Follicular fluid aspirates were collected and oocytes were recovered 34 to 36 hours after hCG injection. The patients proceeded to undergo IVF and ET. Follicular fluid hormones were measured using standard RIA. Angiotensin II and steroid hormone concentrations in FF were compared for pregnant versus nonpregnant women using the Student's t-test and rank-sum test. Pearson multiple-correlation analysis was performed to calculate correlation coefficients among AII concentrations and steroid concentrations in FF aspirates.

RESULTS: Mean FF concentration of AII was significantly lower in samples from women showing clinical pregnancies (112.2 +/- 13.9 pg/mL [107.3 +/- 13.3 pmol/L]) compared with samples from women who did not achieve pregnancy (217.1 +/- 23.8 pg/mL [207.5 +/- 22.7 pmol/L]) (mean +/- SE). A negative correlation was observed between FF concentrations of AII and P. Correlations of AII with E2, T, A, or with ratios of these did not show significance.

CONCLUSION: These data suggest that high AII concentration at time of oocyte recovery may indicate poor pregnancy outcome in women undergoing ovarian stimulation for IVF. These data corroborate previous results in animal models showing that AII predisposes follicles to undergo atresia-like conditions.

Author List

Heimler I, Rawlins RG, Binor Z, Aiman J, Raff H, Hutz RJ

Authors

Reinhold J. Hutz in the Biological Sciences department at University of Wisconsin - Milwaukee
Hershel Raff PhD Professor in the Academic Affairs department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adult
Androstenedione
Angiotensin II
Estradiol
Female
Fertilization in Vitro
Follicle Stimulating Hormone
Follicular Fluid
Humans
Menotropins
Ovulation Induction
Pregnancy
Pregnancy Outcome
Progesterone
Retrospective Studies
Testosterone