Faculty Collaboration Database

Mechanism of interdigestive migrating motor complex in conscious dogs. J Gastroenterol 2010 May;45(5):506-14

Date

12/25/2009

Pubmed ID

20033824

DOI

10.1007/s00535-009-0190-z

Scopus ID

2-s2.0-77953321807 (requires institutional sign-in at Scopus site)   39 Citations

Abstract

BACKGROUND: The migrating motor complex (MMC) is well characterized by the appearance of gastrointestinal contractions in the interdigestive state. This study was designed to clarify the mechanisms of gastric MMC (G-MMC) and intestinal MMC (I-MMC) in conscious dogs.

METHODS: Five strain gauge transducers were implanted on the stomach and intestine. To investigate the correlation between luminal 5-HT and phase III contractions, gastric and duodenal juices were collected during the MMC cycle. The 5-HT concentrations in gastric and duodenal juice were measured by HPLC. To investigate whether luminal 5-HT initiates MMC, 5-HT (10(-8)-10(-6) M, 10 ml) was administered into the duodenum 20 min after gastric phase III. To investigate the involvement of 5-HT(3) or 5-HT(4) receptors in mediating G-MMC and I-MMC, 5-HT(3) antagonists (ondansetron) or 5-HT(4) antagonists (GR 125,487) were infused for 120 min.

RESULTS: Luminal administration of 5-HT (10(-6) M) initiated duodenal phase II followed by G-MMC and I-MMC with a concomitant increased release of plasma motilin. The duodenal 5-HT concentration was significantly increased during phase II (59 +/- 9 ng/ml) and phase III (251 +/- 21 ng/ml) compared to that of phase I (29 +/- 5 ng/ml). On the other hand, the 5-HT content in the stomach was not significantly changed throughout the MMC cycle. Intravenous infusion of motilin (0.3 microg/kg/h) increased the luminal 5-HT content and induced G-MMC and I-MMC. 5-HT(4) antagonists significantly inhibited both G-MMC and I-MMC, while 5-HT(3) antagonists inhibited only G-MMC.

CONCLUSION: It is suggested that the MMC cycle is mediated by a positive feedback mechanism via the interaction between motilin and 5-HT.

Author List

Nakajima H, Mochiki E, Zietlow A, Ludwig K, Takahashi T

Author

Kirk A. Ludwig MD Chief, Professor in the Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Consciousness
Dogs
Duodenum
Female
Gastrointestinal Agents
Jejunum
Motilin
Mucous Membrane
Myoelectric Complex, Migrating
Pyloric Antrum
Serotonin
Serotonin Agents
Transducers, Pressure