Apolipoprotein E inhibition of proliferation of mitogen-activated T lymphocytes: production of interleukin 2 with reduced biological activity. Cell Immunol 1994 Dec;159(2):124-39
Date
12/01/1994Pubmed ID
7994749DOI
10.1006/cimm.1994.1302Scopus ID
2-s2.0-0028135706 (requires institutional sign-in at Scopus site) 120 CitationsAbstract
Apolipoprotein E (apoE), but not apoAI or apoCIII, suppresses mitogen-activated T lymphocyte proliferation, independent of the type of activation signal. Both CD4 and CD8 T cells are inhibited. ApoE inhibits T cell proliferation, in part, by reducing the production of bioactive interleukin 2 (IL2). IL2 activity is reduced by approximately 50-65% in cultures of mitogen-stimulated T cells when apoE is present. ApoE does not significantly alter levels of IL2 mRNA or the mass of secreted IL2 protein, quantitated by enzyme immunoassay. Reduced IL2 activity was not a consequence of induction of IL2 inhibitors in response to apoE or effects of apoE on the bioassay. These results suggest that apoE antagonizes post-translational events in mitogen-activated T lymphocytes that are required for the secretion of a bioactive IL2 protein.
Author List
Kelly ME, Clay MA, Mistry MJ, Hsieh-Li HM, Harmony JAMESH terms used to index this publication - Major topics in bold
Apolipoproteins ECells, Cultured
DNA Replication
Humans
Interleukin-2
Lymphocyte Activation
Phytohemagglutinins
Precipitin Tests
RNA, Messenger
T-Lymphocytes
T-Lymphocytes, Helper-Inducer
T-Lymphocytes, Regulatory
Tetradecanoylphorbol Acetate
