Characterization of a monoclonal antibody against latex protein associated with latex allergy. J Allergy Clin Immunol 1993 Nov;92(5):638-43
Date
11/01/1993Pubmed ID
8227854DOI
10.1016/0091-6749(93)90005-zScopus ID
2-s2.0-0027496312 (requires institutional sign-in at Scopus site) 17 CitationsAbstract
Several hybridomas were produced against antigens extracted from the latex sap of Hevea brasiliensis. One of the monoclonal antibodies (LA-E3) reacted with antigens demonstrating binding to patient sera on crossed enzyme immunoelectrophoresis. This monoclonal antibody reacted with 2 of 10 glove extracts studied and with both Malaysian and Indian latex plant extracts. The antigens purified with monoclonal antibody affinity chromatography demonstrated specific IgE in the sera of patients with latex allergy as determined by ELISA. This monoclonal antibody can thus be utilized to obtain reliable antigens useful in the diagnosis of latex sensitivity, although additional antigens will likely be necessary to enhance sensitivity and specificity.
Author List
Kurup VP, Kumar A, Kelly KJ, Fink JNMESH terms used to index this publication - Major topics in bold
AdolescentAdult
Anaphylaxis
Animals
Antibodies, Monoclonal
Antibody Specificity
Drug Hypersensitivity
Female
Humans
Immunoglobulin E
Latex
Male
Mice
Mice, Inbred BALB C
Plant Proteins
