Faculty Collaboration Database

Modulation of mitochondrial bioenergetics in the isolated Guinea pig beating heart by potassium and lidocaine cardioplegia: implications for cardioprotection. J Cardiovasc Pharmacol 2009 Oct;54(4):298-309

Date

07/22/2009

Pubmed ID

19620879

Pubmed Central ID

PMC2774837

DOI

10.1097/FJC.0b013e3181b2b842

Scopus ID

2-s2.0-74949134558 (requires institutional sign-in at Scopus site)   27 Citations

Abstract

Mitochondria are damaged by cardiac ischemia/reperfusion (I/R) injury but can contribute to cardioprotection. We tested if hyperkalemic cardioplegia (CP) and lidocaine (LID) differently modulate mitochondrial (m) bioenergetics and protect hearts against I/R injury. Guinea pig hearts (n = 71) were perfused with Krebs Ringer's solution before perfusion for 1 minute just before ischemia with either CP (16 mM K) or LID (1 mM) or Krebs Ringer's (control, 4 mM K). The 1-minute perfusion period assured treatment during ischemia but not on reperfusion. Cardiac function, NADH, FAD, m[Ca], and superoxide (reactive oxygen species) were assessed at baseline, during the 1-minute perfusion, and continuously during I/R. During the brief perfusion before ischemia, CP and LID decreased reactive oxygen species and increased NADH without changing m[Ca]. Additionally, CP decreased FAD. During ischemia, NADH was higher and reactive oxygen species was lower after CP and LID, whereas m[Ca] was lower only after LID. On reperfusion, NADH and FAD were more normalized, and m[Ca] and reactive oxygen species remained lower after CP and LID. Better functional recovery and smaller infarct size after CP and LID were accompanied by better mitochondrial function. These results suggest that mitochondria may be implicated, directly or indirectly, in protection by CP and LID against I/R injury.

Author List

Aldakkak M, Stowe DF, Lesnefsky EJ, Heisner JS, Chen Q, Camara AK

Author

Amadou K. Camara PhD Professor in the Anesthesiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Calcium
Cardioplegic Solutions
Cardiotonic Agents
Energy Metabolism
Guinea Pigs
Heart Arrest, Induced
Heart Rate
In Vitro Techniques
Lidocaine
Mitochondria, Heart
Myocardial Contraction
Myocardial Reperfusion Injury
Oxygen
Perfusion
Potassium
Reactive Oxygen Species