Muscarinic M1 modulation of acid-sensing ion channels. Neuroreport 2009 Oct 07;20(15):1386-91
Date
09/05/2009Pubmed ID
19730136DOI
10.1097/WNR.0b013e3283318912Scopus ID
2-s2.0-70349671764 (requires institutional sign-in at Scopus site) 4 CitationsAbstract
Acid-sensing ion channels (ASICs) are ligand-gated cation channels that are highly expressed in nervous system. Little is known about the regulation of these channels. Therefore, we tested whether muscarinic M1 receptors can modulate ASICs. The muscarinic agonist oxotremorine methiodide applied to the bath solution strongly inhibited the whole-cell current in Chinese hamster ovary cells heterologously expressing ASIC1a and M1 receptors. Maximal current was inhibited 30% during muscarinic receptor stimulation. These effects were fast, fully reversible and subunit specific. The acid-sensing current in population of isolated rat hippocampus CA1 and striatum interneurons, thought to be carried primarily by ASIC1a, was similarly inhibited by oxotremorine methiodide. Thus, the current study identifies ASIC1a as a novel target for muscarinic signaling.
Author List
Dorofeeva NA, Karpushev AV, Nikolaev MV, Bolshakov KV, Stockand JD, Staruschenko AMESH terms used to index this publication - Major topics in bold
Acid Sensing Ion ChannelsAnimals
CHO Cells
Corpus Striatum
Cricetinae
Cricetulus
Hippocampus
Interneurons
Ion Channel Gating
Membrane Potentials
Muscarinic Agonists
Nerve Tissue Proteins
Organ Culture Techniques
Oxotremorine
Patch-Clamp Techniques
Rats
Receptor Cross-Talk
Receptor, Muscarinic M1
Sodium Channels
