Faculty Collaboration Database

Interactive effects of volatile anesthetics, verapamil, and ryanodine on contractility and calcium homeostasis of isolated pregnant rat myometrium. Am J Obstet Gynecol 1992 Sep;167(3):804-10

Date

09/01/1992

Pubmed ID

1530042

DOI

10.1016/s0002-9378(11)91593-2

Scopus ID

2-s2.0-0026793980 (requires institutional sign-in at Scopus site)   10 Citations

Abstract

OBJECTIVE: Both volatile anesthetics and Ca2+ antagonists decrease uterine contractility. The interactive effects of anesthetics, verapamil, and ryanodine on myometrial muscular activity and on intracellular Ca2+ availability were examined.

STUDY DESIGN: The effects of minimum alveolar concentrations of 0.5, 1.0, and 2.0 of halothane, enflurane, and isoflurane on electrically stimulated isometric mechanical activity of isolated longitudinal myometrium strips from 45 pregnant rats (15 to 21 days), superfused with Krebs' solution with or without 5.0 mmol/L Ca2+, 10(-7) and 10(-6) mol/L verapamil, and 10(-6) mol/L ryanodine, were compared. Analysis was performed by analysis of variance and Duncan test.

RESULTS: All three anesthetics and verapamil produced dose-dependent depression of contractility. Increasing the Ca2+ concentration from 2.5 to 5.0 mmol/L in the superfusate partly reversed the depression. An additive effect of verapamil and anesthetics, especially enflurane, on contractile force was observed. Ryanodine had no effect on contractility, but it could partly counteract the depressant effect of anesthetics.

CONCLUSION: Clinically used concentrations of volatile anesthetics modify Ca2+ availability and depress uterine contractility. General anesthesia, especially by enflurane, in patients being treated with calcium antagonists may represent a higher risk.

Author List

Laszlo A, Buljubasic N, Zsolnai B, Kampine JP, Bosnjak ZJ

Author

Zeljko Bosnjak PhD, MS Emeritus Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Anesthetics
Animals
Calcium
Dose-Response Relationship, Drug
Drug Interactions
Female
Homeostasis
In Vitro Techniques
Myometrium
Osmolar Concentration
Pregnancy
Rats
Rats, Inbred Strains
Ryanodine
Uterine Contraction
Verapamil