Measuring kidney function in conscious mice. Methods Mol Biol 2009;573:75-94
Date
09/19/2009Pubmed ID
19763923DOI
10.1007/978-1-60761-247-6_5Scopus ID
2-s2.0-73949088547 (requires institutional sign-in at Scopus site) 2 CitationsAbstract
Mice lacking or over-expressing a gene of experimental interest have become important tools to understand the regulation of kidney function and water and electrolyte homeostasis. The use of mice in physiological studies is becoming more widespread, but there are still a number of technical limitations that preclude the full utilization of mouse models in renal research. The present chapter focuses upon a set of methods developed in our laboratory to quantify renal function in conscious mice. These measurements are based upon surgical instrumentation of mice with chronic indwelling arterial and venous catheters. This preparation permits direct measurement of arterial blood pressure, direct sampling of arterial and/or venous blood, intravenous or intra-arterial infusion of substances, and quantification of daily sodium balance. The advantage of these techniques is that all of these procedures can be performed in conscious mice freely moving in their home cages. As such, this in vivo preparation provides an assessment of physiological function in mice in their native state.
Author List
Mattson DLMESH terms used to index this publication - Major topics in bold
Animal HusbandryAnimals
Blood Pressure Determination
Cardiovascular Surgical Procedures
Consciousness
Hormones
Infusions, Intravenous
Kidney Function Tests
Mice
Models, Biological
Sodium
