Faculty Collaboration Database

Expression of the serum response factor gene is regulated by serum response factor binding sites. J Biol Chem 1996 Jul 12;271(28):16535-43

Date

07/12/1996

Pubmed ID

8663310

DOI

10.1074/jbc.271.28.16535

Scopus ID

2-s2.0-0029895008 (requires institutional sign-in at Scopus site)   76 Citations

Abstract

The serum response factor (SRF) is a ubiquitous transcription factor that plays a central role in the transcriptional response of mammalian cells to a variety of extracellular signals. Notably, SRF has been found to be a key regulator of members of a class of cellular response genes termed immediate-early genes (IEGs), many of which are believed to be involved in regulating cell growth and differentiation. The mechanism by which SRF activates transcription of IEGs in response to mitogenic agents has been extensively studied. Significantly less is known about how expression of the SRF gene itself is mediated. We and others have previously shown that the SRF gene is itself transiently induced by a variety of mitogenic agents and belongs to a class of "delayed" early response genes. We have cloned the SRF promoter and in the present study have analyzed the upstream regulatory sequences involved in mediating serum responsiveness of the SRF gene. Our analysis indicates that inducible SRF expression requires both SRF binding sites located within the first 63 nucleotides upstream from the start site of transcriptional initiation and an Sp1 site located 83 nucleotides upstream from the start site. Maximal transcriptional activity of the promoter also requires two CCAATT box sites located 90 and 123 nucleotides upstream of the start site.

Author List

Spencer JA, Misra RP

Author

Ravindra P. Misra PhD Associate Provost, Professor in the Biochemistry department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

3T3 Cells
Animals
Base Sequence
Binding Sites
Blood
DNA
DNA-Binding Proteins
Gene Expression Regulation
Humans
Mice
Molecular Sequence Data
Nuclear Proteins
Promoter Regions, Genetic
Protein Binding
Sequence Homology, Nucleic Acid
Serum Response Factor
Transcription Factors