Faculty Collaboration Database

Linkage mapping of the tumor necrosis factor receptor 2 (TNFR2) gene to 1p36.2 using the single-strand conformation polymorphism technique. Hum Genet 1994 Oct;94(4):418-22

Date

10/01/1994

Pubmed ID

7927340

DOI

10.1007/BF00201604

Scopus ID

2-s2.0-0028071077 (requires institutional sign-in at Scopus site)   10 Citations

Abstract

We identified two informative polymorphisms in the transcribed 3' untranslated region of the tumor necrosis factor receptor 2 (TNFR2) gene using the polymerase chain reaction (PCR) with the single-strand conformation polymorphism (SSCP) technique. These polymorphisms demonstrated Mendelian inheritance and were useful for linkage analysis. TNFR2 was very closely linked to the pronatriodilatin gene (PND), and the TNFR2 SSCP polymorphisms were much more informative than the restriction fragment length polymorphisms currently available for the PND locus. In addition, we have demonstrated that genotyping could be performed with DNA obtained from paraffin-embedded tissue.

Author List

Kaufman BA, White PS, Steinbrueck T, Donis-Keller H, Brodeur GM

MESH terms used to index this publication - Major topics in bold

Base Sequence
Chromosome Mapping
Chromosomes, Human, Pair 1
Genetic Linkage
Humans
Molecular Sequence Data
Paraffin Embedding
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Receptors, Tumor Necrosis Factor