Novel method to quantify neuropil threads in brains from elders with or without cognitive impairment. J Histochem Cytochem 2000 Dec;48(12):1627-38
Date
12/02/2000Pubmed ID
11101631DOI
10.1177/002215540004801206Scopus ID
2-s2.0-0033639241 (requires institutional sign-in at Scopus site) 70 CitationsAbstract
Pathological alterations in dendrites and axons (i.e., neuritic pathologies) occur in the normal aging brain as well as in brains from elders with mild cognitive impairment and neurodegenerative dementia. These alterations may correlate with clinical measures of cognitive abilities, but the contribution of neuropil threads (NTs), which constitute 85-90% of cortical tau pathology, has not been clear because of the lack of quantitative methodologies. We combined quantitative fractionation and image analysis to devise a strategy for measuring the burden of tau-rich NTs in the entorhinal and perirhinal cortex of brains from elders with and without cognitive impairment, including dementia due to Alzheimer's disease (AD). On the basis of data presented here using this novel strategy, we conclude that this quantitative imaging technique will facilitate efforts to determine the behavioral correlations of neuritic lesions in AD and other brain disorders.
Author List
Mitchell TW, Nissanov J, Han LY, Mufson EJ, Schneider JA, Cochran EJ, Bennett DA, Lee VM, Trojanowski JQ, Arnold SEAuthor
Elizabeth J. Cochran MD Adjunct Professor in the Pathology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AgedAged, 80 and over
Alzheimer Disease
Brain
Chemical Fractionation
Cognition Disorders
Dementia
Humans
Image Processing, Computer-Assisted
Immunohistochemistry
Neuropil Threads
