Alpha 1-antichymotrypsin is present in and synthesized by the cornea. Curr Eye Res 1994 Jun;13(6):433-9
Date
06/01/1994Pubmed ID
7924407DOI
10.3109/02713689408999871Scopus ID
2-s2.0-0028338811 (requires institutional sign-in at Scopus site) 17 CitationsAbstract
The proteinase inhibitor alpha 1-antichymotrypsin is present in the epithelial, stromal and endothelial layers of the human cornea. This was determined by immunolocalization in corneal sections and by Western blot analysis of extracts from the three separated layers. The inhibitor was quantified in the extracts by immunodot blot analysis. The levels observed were 1.3 +/- 0.3 microgram/cornea for the epithelial layer, 22.8 +/- 3.8 micrograms/cornea for the stromal layer and an average of 0.17 micrograms/cornea for the endothelial layer. alpha 1-Antichymotrypsin is being synthesized by the cornea. Metabolically labeled inhibitor was immunoprecipitated from the three layers following organ culture of the intact cornea. Two major forms were detected. These were the native, mature 64 kDa form and a 50 kDa form which is either a degradation product or an incompletely glycosylated form. These results indicate that the cornea has the ability to locally control degradation through synthesis of this inhibitor. Local synthesis of this inhibitor releases the cornea from total dependance upon the vascular system for its supply of alpha 1-antichymotrypsin.
Author List
Twining SS, Fukuchi T, Yue BY, Wilson PM, Zhou XAuthor
Sally S. Twining PhD Assistant Dean, Professor in the Biochemistry department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AdolescentAdult
Aged
Aged, 80 and over
Blotting, Western
Child, Preschool
Cornea
Corneal Stroma
Electrophoresis, Polyacrylamide Gel
Endothelium, Corneal
Epithelium
Humans
Immunoenzyme Techniques
Middle Aged
Molecular Weight
Organ Culture Techniques
Precipitin Tests
alpha 1-Antichymotrypsin
