Vasoactive intestinal polypeptide antiserum affects rat prolactin mRNA in 40-day but not 110-day diethylstilbestrol-induced prolactinoma tissue. Mol Cell Neurosci 1994 Apr;5(2):182-8
Date
04/01/1994Pubmed ID
8032686DOI
10.1006/mcne.1994.1020Scopus ID
2-s2.0-0028198434 (requires institutional sign-in at Scopus site) 1 CitationAbstract
Experiments were designed to examine whether vasoactive intestinal polypeptide (VIP), a known stimulator of basal prolactin (PRL) secretion, regulates PRL gene expression in the rat diethylstilbestrol (DES)-induced prolactinoma model. The VIP-induced increase in PRL release could result from increased PRL synthesis and/or decreased PRL degradation. Male Fischer 344 rats were implanted with 10 mg DES pellets 40 or 110 days prior to obtaining the anterior pituitary glands for cell dispersal. Cells were incubated in 1:10 normal rabbit serum or VIP antiserum (AVIP). After incubation, cells were pelleted, washed, and pooled for total nucleic acid extraction. The rat PRL (rPRL) mRNA abundance was quantitated using a solution hybridization/ribonuclease protection assay. Supernatant was collected and analyzed for PRL content using radioimmunoassay. Results from this experiment reveal partial immunoneutralization of intrapituitary VIP significantly decreased PRL secretory rate by rapid reduction in rRPL mRNA in the 40-day tumors. However, in the 110-day tumors the rPRL mRNA steady-state levels were unchanged but the basal release of PRL continued to be decreased by AVIP. These results indicate VIP exerts its effects on PRL secretion through at least two mechanisms.
Author List
Maas DL, Meier DA, Wahle JS, Martinson DR, Hagen TCMESH terms used to index this publication - Major topics in bold
AnimalsDiethylstilbestrol
Immune Sera
Male
Pituitary Neoplasms
Prolactin
Prolactinoma
RNA, Messenger
Rats
Rats, Inbred F344
Recombinant Proteins
Time Factors
Vasoactive Intestinal Peptide
