Faculty Collaboration Database

Gene expression and transcript size of the prepro-peptide VIP/PHM-27 in normal human tissue. Neurosci Lett 1993 Dec 24;164(1-2):101-4

Date

12/24/1993

Pubmed ID

8152582

DOI

10.1016/0304-3940(93)90867-k

Scopus ID

2-s2.0-0027722982 (requires institutional sign-in at Scopus site)   5 Citations

Abstract

The transcript size of VIP/PHM-27 mRNA (vasoactive intestinal peptide/peptide histidine methionine) and the relative distribution of VIP/PHM-27 gene expression in 10 normal human tissues was examined. After mRNA extraction from tissue, VIP/PHM-27 transcript size and relative abundance of mRNA was determined by Northern blot analysis and densitometry of the autoradiograms. VIP/PHM-27 mRNA was detectable in brain, pancreas, colon, ileum and striated muscle while no hybridization signal was observed in liver, kidney, lung, heart, prostate and placental tissue. VIP/PHM-27 transcript in human brain and gut was a single band of 1.7 kb; by contrast, a 7.0-kb transcript was detected in striated skeletal muscle. The highest relative levels of mRNA were observed in brain and pancreas.

Author List

Schulte-Bockholt A, Meier DA, Hopp K, Stadelmann A, Otterson MF, Telford GL, Koch TR

Author

Mary F. Otterson MS, MD Emeritus Professor in the Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Autoradiography
Blotting, Northern
Exons
Gene Expression
Humans
In Situ Hybridization
Peptide PHI
Protein Precursors
RNA, Messenger
Rats
Transcription, Genetic
Vasoactive Intestinal Peptide