Faculty Collaboration Database

The role of microfilaments in the capping of epidermal growth factor receptor in A431 cells. Exp Cell Res 1991 May;194(1):48-55

Date

05/01/1991

Pubmed ID

1901794

DOI

10.1016/0014-4827(91)90128-h

Scopus ID

2-s2.0-0026164770 (requires institutional sign-in at Scopus site)   13 Citations

Abstract

Capping of the EGF receptor (EGF-R) on the surface of suspended and adherent epidermoid carcinoma cells, A431, is studied. It was induced at 20 degrees C after treating cells with monoclonal antibody to the EGF receptor followed by the second antibody conjugated with FITC. Accumulation of cortical actin under the caps was detected by rhodamine-phalloidin. Destruction of the actin stress-fiber-like bundles was observed during incubation of cells with the ligands at 0 degrees C. Two processes appear to take place at 20 degrees C: redistribution of the EGF-R with cortical actin into the caps within 15-30 min and reconstruction of cytoplasmic actin bundles over 45-60 min. Dihydrocytochalasin B prevented cap formation in adherent cells, but small patches of EGF-R colocalized with actin aggregates under plasma membrane were observed. The function of different actin-containing cytoskeleton structures in the process of capping is discussed.

Author List

Khrebtukova IA, Kwiatkowska K, Gudkova DA, Sorokin AB, Pinaev GP

Author

Andrey Sorokin PhD Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Actin Cytoskeleton
Actins
Antibodies, Monoclonal
Carcinoma, Squamous Cell
Cell Membrane
Cytochalasin B
ErbB Receptors
Fluorescein-5-isothiocyanate
Fluoresceins
Fluorescent Antibody Technique
Humans
Receptor Aggregation
Thiocyanates
Tumor Cells, Cultured