[The capping of receptors for the epidermal growth factor and the participation of the cytoskeleton in this process in A-431 cells]. Tsitologiia 1989 Oct;31(10):1211-20
Date
10/01/1989Pubmed ID
2617672Scopus ID
2-s2.0-0024351604 (requires institutional sign-in at Scopus site) 1 CitationAbstract
Epidermal growth factor (EGF) receptor capping results from the interaction between the receptors and polyvalent ligands in A-431 cells examined in suspension at 22 degrees C. Colocalization of actin and spectrin with the ligand-receptor complexes during the redistribution was shown using double immunofluorescence. The obtained data show that the cortical microfilaments are involved in capping. EGF receptors become associated with the Triton-insoluble cytoskeleton as a consequence of ligand binding. EGF-receptor capping is not sensitive to the action of cytochalasin B. Capping in A-431 cells is discussed as a new model for studying the redistribution of the ligand-receptor complex.
Author List
Khrebtukova IA, Nikol'skaia AN, Sorkin AD, Sorokin AB, Pinaev GPAuthor
Andrey Sorokin PhD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
ActinsAntibodies
Antibodies, Monoclonal
Carcinoma, Squamous Cell
Cell Line
Cytochalasin B
Cytoskeleton
Epidermal Growth Factor
ErbB Receptors
Humans
Immune Sera
Ligands
Receptor Aggregation
Spectrin
Tumor Cells, Cultured
