Faculty Collaboration Database

Recycling of epidermal growth factor-receptor complexes in A431 cells. Biochim Biophys Acta 1989 Mar 28;1011(1):88-96

Date

03/28/1989

Pubmed ID

2784329

DOI

10.1016/0167-4889(89)90083-9

Scopus ID

2-s2.0-0024581749 (requires institutional sign-in at Scopus site)   55 Citations

Abstract

The fate of epidermal growth factor (EGF) after internalization by human carcinoma A431 cells has been studied. Cells were allowed to internalize 125I-EGF for 10 min at 37 degrees C and treated with acid/salt solution to remove non-internalized ligand. Further incubation of these '125I-EGF-loaded' cells at 37 degrees C results in rapid recycling of internalized 125I-EGF-receptor complexes back to the cell surface. Recycling was assessed by measuring the increase in plasma membrane pool of 125I-EGF-receptor complexes as they became sensitive to acid/salt treatment, cross-linking with the membrane impermeant reagent bis(sulfosuccinimidyl)suberate and competitive substitution by unlabeled EGF. Moreover, redistribution of 125I-EGF-receptor complexes from endosomes to the plasma membrane was demonstrated using a subcellular fractionation technique. More than 50% of the total internalized EGF was found to be capable of recycling. The rate of recycling was significantly higher than that of EGF degradation in lysosomes. It was shown that EGF/receptor recycling is an energy-requiring and temperature-dependent process. Fluorescence microscopy studies demonstrate that endosomes located in a region adjacent to the Golgi complex are involved in the the recycling of EGF-receptor complexes in A431 cells. The data obtained suggest that dissociation of EGF from internalized receptor is not necessary for EGF receptor recycling.

Author List

Sorkin A, Kornilova E, Teslenko L, Sorokin A, Nikolsky N

Author

Andrey Sorokin PhD Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Binding, Competitive
Cell Fractionation
Cell Membrane
Cross-Linking Reagents
Energy Metabolism
Epidermal Growth Factor
ErbB Receptors
Fluorescent Dyes
Golgi Apparatus
Humans
Kinetics
Mice
Microscopy, Fluorescence
Succinimides
Temperature
Tumor Cells, Cultured