[The recycling of EGF-receptor complexes in A431 cells]. Tsitologiia 1989 Mar;31(3):300-11
Date
03/01/1989Pubmed ID
2787557Scopus ID
2-s2.0-0024524207 (requires institutional sign-in at Scopus site) 1 CitationAbstract
As was demonstrated elsewhere (L. V. Teslenko et al., 1987), the epidermal growth factor (EGF) can recycle after internalization by A431 cells in membrane-bound state. In the present study, direct evidence on recycling of EGF-receptor complexes is presented using a covalently crosslinking reagent. The recycling was shown to occur via peripheral endosomes as well as through para-Golgi endosomes. It was found that among EGF degradation inhibitors tested only primaquine (300 microM) was able to decrease significantly the rate of recycling. The lowering of the temperature to 17 degrees C led to blocking the EGF degradation as well as to inhibiting the recycling. The data obtained suggested that the recycling of EGF-receptor complexes is relatively independent of their degradation.
Author List
Sorkin AD, Bogdanova NP, Sorokin AB, Teslenko LV, Nikol'skiĭ NNAuthor
Andrey Sorokin PhD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCell Compartmentation
Cell Line
Epidermal Growth Factor
ErbB Receptors
Humans
Iodine Radioisotopes
Mice
Organelles
Rhodamines
Temperature
Tumor Cells, Cultured
