Medical College of Wisconsin
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[Dependence of the proliferation of cell line 3T6 on a totally serum-free medium on epidermal growth factor]. Tsitologiia 1987 Nov;29(11):1309-13

Date

11/01/1987

Pubmed ID

3326232

Scopus ID

2-s2.0-0023446152 (requires institutional sign-in at Scopus site)

Abstract

The Eagle medium containing the epidermal growth factor (EGF), insulin, transferrin and a source of iron is able to support proliferation of 3T6 cells in the absence of serum. The formation of 3T6 cell clones in the similar medium supplemented with non-essential amino acids confirms the usefulness of such a medium for cell cultivation. It has been found that the exclusion of EGF rather than other above components from the medium inhibits most highly the increase in cell number.

Author List

Gudkova DA, Sorokin AB

Author

Andrey Sorokin PhD Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Amino Acids
Animals
Cell Division
Cell Line
Cells, Cultured
Culture Media
Epidermal Growth Factor
Immune Sera
Insulin
Iron
Time Factors
Transferrin