[Effect of cultivation conditions on the growth properties of Swiss 3T3 cells]. Tsitologiia 1984 Oct;26(10):1161-7
Date
10/01/1984Pubmed ID
6515717Scopus ID
2-s2.0-0021745197 (requires institutional sign-in at Scopus site)Abstract
A possibility of using Swiss 3T3 cells, adapted to the growth in the Eagle basal medium and bovine serum, in studies of cell proliferation and quiescent state was shown on the basis of their growth characteristics. Proliferative activity of cultures was estimated by measuring the intensity of DNA synthesis (incorporation of labeled thymidine and flow cytofluorometric analysis), mitotic index and cell number counts. Growth rate and saturation density of the culture were analyzed depending on serum concentration, substrate quality and medium changes both in growth and quiescent states. In spite of repeated medium changes such adapted cells had saturation density within 4.10(4)--7.10(4) cells/cm2, standard for this line. Besides, a distinct inhibition of cell proliferation at confluence or after incubation with low serum (0.5%) and a possibility of the following stimulation of cell divisions by adding a fresh medium containing different concentrations of serum were demonstrated. The increased rate of adipose conversion was detected in resting confluent 3T3 cells cultivated in closed vessels, as compared to cells growing in tissue culture dishes in the CO2 incubator.
Author List
Barkan RS, Zenin VV, Skopicheva VI, Sorokin ABAuthor
Andrey Sorokin PhD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCell Count
Cell Division
Cell Line
Cells, Cultured
Culture Media
DNA
Mice
Mice, Inbred Strains
Mitosis
Time Factors
