Faculty Collaboration Database

Characterization of Micropolyspora faeni antigens. Infect Immun 1981 Nov;34(2):508-12

Date

11/01/1981

Pubmed ID

6171521

Pubmed Central ID

PMC350896

DOI

10.1128/iai.34.2.508-512.1981

Scopus ID

2-s2.0-0019769897 (requires institutional sign-in at Scopus site)   8 Citations

Abstract

Culture filtrate antigens of Micropolyspora faeni grown in a synthetic medium in a stirred fermentor were characterized. The culture filtrate antigens were fractionated by preparative isoelectric focusing with a pH gradient of 3.5 to 5.5. The fractions were pooled according to their reaction with rabbit anti-M. faeni sera. A pool containing two major antigens which were resolved by analytical isoelectric focusing and polyacrylamide disc gel electrophoresis was obtained. One antigen was stainable with Coomassie blue and periodic acid-Schiff stain and was determined to have a mass of 51,000 daltons. The other antigen was stainable only with Coomassie blue and was determined to have a mass of 29,000 daltons. When used at 1 mg/ml, this pool reacted with the sera from all patients with farmer's lung disease by immunodiffusion but failed to react with control sera.

Author List

Kurup VP, Ting EY, Fink JN, Calvanico NJ

MESH terms used to index this publication - Major topics in bold

Antigens, Bacterial
Bacterial Proteins
Electrophoresis, Polyacrylamide Gel
Farmer's Lung
Humans
Immunodiffusion
Isoelectric Focusing
Micromonosporaceae
Molecular Weight
Periodic Acid-Schiff Reaction
Staining and Labeling