Guanosine 3',5'-cyclic monophosphate as a mediator of inhibition of renin release. Am J Physiol 1988 Sep;255(3 Pt 2):F474-8
Date
09/01/1988Pubmed ID
2901230DOI
10.1152/ajprenal.1988.255.3.F474Scopus ID
2-s2.0-0023751848 (requires institutional sign-in at Scopus site) 82 CitationsAbstract
The role of guanosine 3',5'-cyclic monophosphate (cGMP) as an inhibitory mediator of tissue renin release was examined in two different in vitro preparations. In rat superficial cortical slices, renin release stimulated by isoproterenol (10(-5) M) was ablated by atriopeptin III (ANP, 2.1 x 10(-8) M), nitroprusside (NP, 10(-3) M), and 8-bromoguanosine 3',5'-cyclic monophosphate (8-BrcGMP, 10(-3) and 10(-6) M). Arachidonic acid (10(-3) M)-stimulated renin release was also inhibited by ANP and 8-BrcGMP (10(-3) and 10(-6) M). Both ANP and NP increased tissue cGMP concentrations significantly (P less than 0.05), but neither had an effect on adenosine 3',5'-cyclic monophosphate (cAMP) concentrations. When methylene blue (10(-5) M), an inhibitor of guanylate cyclase, was added to slices incubated with isoproterenol and ANP, the inhibition of renin release by ANP was abolished. These results were confirmed in a preparation of isolated cultured rat juxtaglomerular cells. In these cells, isoproterenol induced a significant increase (58%, P less than 0.01) in renin release, which was inhibited by the addition of 8-BrcGMP (10(-6) M). These data demonstrate a direct inhibitory effect of ANP on isoproterenol- and arachidonic acid-induced renin release. The results with NP, 8-BrcGMP, and methylene blue suggest that cGMP is an intracellular mediator of this inhibition.
Author List
Henrich WL, McAllister EA, Smith PB, Campbell WBAuthor
William B. Campbell PhD Professor in the Pharmacology and Toxicology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsArachidonic Acid
Arachidonic Acids
Atrial Natriuretic Factor
Cyclic GMP
Guanylate Cyclase
In Vitro Techniques
Isoproterenol
Kidney Cortex
Kinetics
Methylene Blue
Nitroprusside
Rats
Renin
