Immunochemical characterization of Aspergillus fumigatus antigens. Infect Immun 1983 Aug;41(2):698-701
Date
08/01/1983Pubmed ID
6347893Pubmed Central ID
PMC264698DOI
10.1128/iai.41.2.698-701.1983Scopus ID
2-s2.0-0020615678 (requires institutional sign-in at Scopus site) 31 CitationsAbstract
Culture filtrate antigens of Aspergillus fumigatus Ag 534 were purified by preparative isoelectric focusing and affinity chromatography. One of the pooled antigen fractions from the preparative isoelectric focusing step (pool 2) was passed through a concanavalin A column and yielded two components, designated antigens IIa and IIb. Antigen IIb reacted more strongly than antigen IIa with all of the aspergilloma and allergic bronchopulmonary aspergillosis sera tested by enzyme-linked immunosorbent assay. The glycoprotein nature of antigen IIb was shown by the concanavalin A binding properties and staining reactions of the components.
Author List
Kurup VP, Ting EY, Fink JNMESH terms used to index this publication - Major topics in bold
AnimalsAntigens, Fungal
Aspergillosis
Aspergillosis, Allergic Bronchopulmonary
Aspergillus fumigatus
Enzyme-Linked Immunosorbent Assay
Humans
Immunization
Immunochemistry
Isoelectric Focusing
Rabbits
