Cell-mediated immunity to soluble and particulate inhaled antigens. Clin Exp Immunol 1979 Nov;38(2):332-41
Date
11/01/1979Pubmed ID
393444Pubmed Central ID
PMC1537881Scopus ID
2-s2.0-0018595589 (requires institutional sign-in at Scopus site) 4 CitationsAbstract
In order to determine the influence of an antigen's physical properties on the development of cell-mediated immunity (CMI) in the lung following aerosol immunization, human serum albumin (HSA) was prepared in either a soluble or a particulate form, the latter being coupled to respirable, carboxylated latex beads. Antigen was administered via an aerosol to groups of guinea-pigs, twice weekly for up to 4 weeks. Additional groups of animals served as unexposed and unconjugated latex controls. Lymphoid cells for CMI assays were isolated from the lung by bronchopulmonary lavage and from blood for use in mitogen- and antigen-induced lymphocyte transformation assays, as well as indirect macrophage migration inhibition tests. Particulate HSA-exposed animals yielded the highest numbers of free lung cells containing predominantly macrophages, with up to 33% lymphocytes. These were followed by the latex control, soluble HSA and unexposed control groups, respectively. Only the animals exposed to particulate HSA had evidence of antigen reactivation in the lung cell populations as measured by lymphocyte stimulation assays. In contrast, a response to polyclonal mitogens was found only in animals exposed to antigen in a soluble form. Data from macrophage depletion experiments suggest that the antigenicity of inhaled antigens may be due to the types and numbers of cells responding to the stimulus, and the subsequent role the alveolar macrophage may play in the modulation of cellular immunity.
Author List
Hill JO, Burrell RMESH terms used to index this publication - Major topics in bold
AerosolsAnimals
Antigens
Cell Division
Cell Separation
Concanavalin A
Guinea Pigs
Humans
Immunity, Cellular
Latex
Lymphocyte Activation
Lymphocytes
Macrophage Migration-Inhibitory Factors
Macrophages
Microspheres
Phytohemagglutinins
Serum Albumin
Solubility
Therapeutic Irrigation
