In vitro and in vivo inhibition of renin by fatty acids. Am J Physiol 1978 Jun;234(6):E593-9
Date
06/01/1978Pubmed ID
665762DOI
10.1152/ajpendo.1978.234.6.E593Scopus ID
2-s2.0-0017978161 (requires institutional sign-in at Scopus site) 11 CitationsAbstract
Circulating neutral lipids inhibit the in vitro renin reaction. To identify the inhibitor(s), free fatty acids were added to human renin and homologous substrate. Capric, lauric, palmitoleic, linoleic, and arachidonic acids each inhibited the rate of angiotensin I production in vitro (P less than 0.01). Inhibition by polysaturated fatty acids (linoleic and arachidonic) was less (P less than 0.01) after catalytic hydrogenation of the double bonds. To evaluate an in vivo effect of renin inhibition intra-arterial blood pressure responses to infusions of renin and angiotensin II (5.0 microgram) were measured in anephric rats (n = 6) before and after infusion of linoleic acid (10 mg iv). Mean increase of blood pressure to angiotensin II before (75 mmHg +/- 9) and after (90 +/- 12) linoleic acid did not differ (P greater than 0.05). However, the pressor response to renin after linoleic acid (18 +/- 3) was less (P less than 0.00)) than that before (102 +/- 13). In summary, several fatty acids inhibit the in vitro renin reaction, and in part inhibition is dependent on unsaturation. Linoleic acid also inhibits the in vivo pressor response to renin. These results suggest that fatty acids may modify the measurement of plasma renin activity and may also affect angiotensin production in vivo.
Author List
Kotchen TA, Welch WJ, Talwalkar RTMESH terms used to index this publication - Major topics in bold
Angiotensin IAngiotensin II
Animals
Arachidonic Acids
Blood Pressure
Fatty Acids, Nonesterified
Kinetics
Linoleic Acids
Male
Rats
Substrate Specificity
