Faculty Collaboration Database

Determination of experimental myocardial infarct size. J Pharmacol Methods 1981 Nov;6(3):199-210

Date

11/01/1981

Pubmed ID

7329069

DOI

10.1016/0160-5402(81)90109-1

Scopus ID

2-s2.0-0019847146 (requires institutional sign-in at Scopus site)   80 Citations

Abstract

Myocardial infarction was produced in anesthetized dogs by a 2-hr occlusion and 30-min reperfusion of the left anterior descending cornary artery. A balloon-reservoir perfusion system was used for reperfusion and delineation of perfusion bed size (area at risk) with Patent blue dye. Infarct mass was determined by a histochemical staining technique with triphenyl tetrazolium chloride. Regional myocardial perfusion in infarcted, ischemic, and normal regions was measured with radioactive microspheres. Infarct size was 8.5 +/- 1.6 g and accounted for irreversible damage in 9.2 +/- 1.9% and 32.5 +/- 4.8% of the left ventricle and area at risk, respectively. Regional myocardial blood flow within infarcted regions was constant over the 2-hr occlusion period (0.10 +/- 0.03 to 0.11 +/- 0.02 ml/min/g). Following reperfusion, these areas demonstrated significantly lower flow than did normal regions, Ischemic but noninfarcted tissue also had no change in flow over the occlusion period, but flow returned to normal following reperfusion. This study describes reliable methodology for production and determination of infarct size with simultaneous measurement of several factors involved in the relative extent of irreversible tissue damage.

Author List

Warltier DC, Zyvoloski MG, Gross GJ, Hardman HF, Brooks HL

MESH terms used to index this publication - Major topics in bold

Animals
Blood Flow Velocity
Blood Pressure
Coronary Circulation
Coronary Disease
Dogs
Heart Rate
Hemodynamics
Male
Myocardial Infarction
Perfusion