Faculty Collaboration Database

Tissue-specific expression of the K-ras allele from the A/J parent in (A/J x TSG-p53) F1 mice. Gene 1997 Apr 01;188(2):261-9

Date

04/01/1997

Pubmed ID

9133601

DOI

10.1016/s0378-1119(96)00821-9

Scopus ID

2-s2.0-0030999105 (requires institutional sign-in at Scopus site)   13 Citations

Abstract

Tissue-specific expression of parental K-ras allele(s) was investigated by single-strand conformation polymorphism analysis of the 3' untranslated region of the K-ras gene in normal lung, spleen, liver and kidney from (A/J x TSG-p53) F1 mice. The expression of A/J K-ras allele was equal to that of C57BL/6J allele in normal spleen, liver and kidney. However, transcripts from A/J K-ras allele were found to be 2-12-times greater than those from C57BL/6J allele in lung tissues harvested over a 20-week period. Similar to our previous observation with dimethylnitrosamine- and benzo[a] pyrene-induced lung tumors, K-ras mRNA transcribed from A/J allele was 10-40-times more abundant than those from C57BL/6J allele in all of 40 (A/J x TSG-p53) F1 mouse lung tumors induced by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone. In addition, K-ras mutations (G to A transitions at the second base of codon 12) were detected in 38 of 40 (95%) lung tumors and all of the mutations were found on the allele inherited from the A/J parent. These data demonstrate tissue-specific allele-specific transcription of the K-ras gene and provide further support to the thesis that K-ras allele itself is a primary mouse lung tumor susceptibility gene.

Author List

Matzinger SA, Chen B, Wang Y, Crist KA, Stoner GD, Kelloff GJ, Lubet RA, You M

MESH terms used to index this publication - Major topics in bold

Alleles
Animals
Base Sequence
DNA
Female
Gene Expression Regulation
Genes, ras
Kidney
Liver
Lung
Male
Mice
Mice, Inbred Strains
Molecular Sequence Data
Polymorphism, Genetic
Species Specificity
Spleen