Use of AFLP markers for gene mapping and QTL detection in the rat. Genomics 1996 Nov 01;37(3):289-94
Date
11/01/1996Pubmed ID
8938440DOI
10.1006/geno.1996.0562Scopus ID
2-s2.0-0030297617 (requires institutional sign-in at Scopus site) 57 CitationsAbstract
The AFLP technique is a new DNA marker technology based on the selective amplification of restriction fragments. Multiple polymorphic markers are simultaneously produced and can be tested in one PCR. No prior information on genomic DNA sequences is needed. In the current study, we contribute 18 AFLP markers to the linkage map of the rat. Seven AFLP markers were assigned to specific chromosomes by analysis of a (BN x ACI)F1 x ACI backcross progeny. Another 11 AFLP markers were mapped by using a panel of the H x B/B x H recombinant inbred (RI) strains. Genotypes of these AFLP markers were also tested for correlations with some blood pressure phenotypes in the RI strains. Suggestive correlation was found between the mean arterial pressure and two closely linked AFLP markers located on chromosome 20. The current study illustrates the value of AFLP markers for the construction of linkage maps and the detection of quantitative trait loci.
Author List
Otsen M, den Bieman M, Kuiper MT, Pravenec M, Kren V, Kurtz TW, Jacob HJ, Lankhorst A, van Zutphen BFMESH terms used to index this publication - Major topics in bold
AnimalsBlood Pressure
Chromosome Mapping
Crosses, Genetic
Female
Genetic Markers
Male
Phenotype
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Rats
Rats, Inbred ACI
Rats, Inbred BN
