Faculty Collaboration Database

Expression of mRNA for vasoactive intestinal peptide in rat small intestine. Mol Cell Endocrinol 1996 Jan 15;116(1):31-7

Date

01/15/1996

Pubmed ID

8822262

DOI

10.1016/0303-7207(95)03693-8

Scopus ID

2-s2.0-0030034419 (requires institutional sign-in at Scopus site)   10 Citations

Abstract

Transplantation of small intestine is a neural model that permits studies of expression of the neuropeptide, vasoactive intestinal peptide, following extrinsic denervation, transection of intrinsic neural pathways, and an ischemic interval. Tissue levels of vasoactive intestinal peptide were examined at 3 months in ileum from a sham operation, in ileum after resection of proximal small intestine, in ileum after resection of proximal small intestine and extrinsic denervation, in ileum after resection of proximal small intestine and 30 min of ischemia, and in ileum obtained 3 months after ileal isografting in Lewis-to-Lewis combinations. Vasoactive intestinal peptide levels were increased in transplanted rat ileum, resection controls, denervation controls, and ischemic controls compared to sham-operated ileum (pANOVA < 0.01). The increased levels of this peptide were highest in denervation controls and lowest in ischemic controls. Northern blot analysis using rat vasoactive intestinal peptide cDNA identified a single 1.7-kb transcript in normal and transplanted rat ileum. The density of vasoactive intestinal peptide transcripts was increased in transplanted ileum (8450 +/- 540) compared to normal ileum (5790 +/- 620) (P < 0.01), and the ratio of this transcript to glyceraldehyde-3-phosphate dehydrogenase density units was also increased in transplanted ileum (0.81 +/- 0.08) compared to normal ileum (0.40 +/- 0.07; P < 0.01). Enhanced transcriptional regulation was the likely mechanism for increased tissue vasoactive intestinal peptide. The increased tissue levels appeared to be a response to extrinsic denervation and transection of intrinsic neural pathways, while an ischemic interval appeared to decrease tissue levels of the peptide.

Author List

Stadelmann AM, Telford GL, Appel DA, Walgenbach-Telford S, Hopp K, Meier DA, Koch TR

MESH terms used to index this publication - Major topics in bold

Animals
Denervation
Gene Expression Regulation
Intestine, Small
Ischemia
RNA, Messenger
Radioimmunoassay
Rats
Rats, Inbred Lew
Transplantation, Isogeneic
Up-Regulation
Vasoactive Intestinal Peptide