Faculty Collaboration Database

Transforming growth factor-alpha overexpression in proliferative verrucous leukoplakia and oral squamous cell carcinoma: an immunohistochemical study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1996 Jul;82(1):69-74

Date

07/01/1996

Pubmed ID

8843456

DOI

10.1016/s1079-2104(96)80379-9

Scopus ID

2-s2.0-0030184836 (requires institutional sign-in at Scopus site)   37 Citations

Abstract

Proliferative verrucous leukoplakia is a unique type of oral leukoplakia that has a high risk of malignant transformation. The aim of this study was to examine the expression of transforming growth factor-alpha in proliferative verrucous leukoplakia, oral squamous cell carcinoma, and normal mucosa. Transforming growth factor-alpha, a potent mitogen, is known to play an important role in various neoplasms including oral squamous cell carcinoma. Immunohistochemical localization of transforming growth factor-alpha in archival paraffin-embedded sections was performed with commercially available monoclonal antibodies. Ten cases each of normal mucosa, proliferative verrucous leukoplakia, and oral squamous cell carcinoma were stained. Quantification of the staining intensity, expressed as the cytoplasmic optical density, was done with the Roche Image Analysis System. The data were statistically analyzed with the one-way analysis of variance and Tukey tests. Notably, the mean cytoplasmic optical density of proliferative verrucous leukoplakia was significantly higher than the mean cytoplasmic optical density of normal mucosa (p < 0.01). The mean cytoplasmic optical density of proliferative verrucous leukoplakia was slightly higher than that of oral squamous cell carcinoma, however, this difference was not significant (p > 0.05). The mean cytoplasmic optical density values demonstrate that increased transforming growth factor-alpha immunoreactivity occurs in proliferative verrucous leukoplakia and oral squamous cell carcinoma relative to normal mucosa.

Author List

Kannan R, Bijur GN, Mallery SR, Beck FM, Sabourin CL, Jewell SD, Schuller DE, Stoner GD

MESH terms used to index this publication - Major topics in bold

Adult
Aged
Aged, 80 and over
Analysis of Variance
Biomarkers, Tumor
Carcinoma, Squamous Cell
Case-Control Studies
Cell Count
Cell Transformation, Neoplastic
Densitometry
Female
Humans
Immunoenzyme Techniques
Leukoplakia, Oral
Male
Middle Aged
Mouth Mucosa
Mouth Neoplasms
Retrospective Studies
Sex Ratio
Transforming Growth Factor alpha