Gene targeting in the rat: advances and opportunities. Trends Genet 2010 Dec;26(12):510-8
Date
09/28/2010Pubmed ID
20869786Pubmed Central ID
PMC2991520DOI
10.1016/j.tig.2010.08.006Scopus ID
2-s2.0-78549295495 (requires institutional sign-in at Scopus site) 91 CitationsAbstract
The rat has long been a model favored by physiologists, pharmacologists and neuroscientists. However, over the past two decades, many investigators in these fields have turned to the mouse because of its gene modification technologies and extensive genomic resources. Although the genomic resources of the rat have nearly caught up, gene targeting has lagged far behind, limiting the value of the rat for many investigators. In the past two years, advances in transposon- and zinc finger nuclease (ZFN)-mediated gene knockout as well as the establishment and culturing of embryonic and inducible pluripotent stem cells have created new opportunities for rat genetic research. Here, we provide a high-level description and the potential uses of these new technologies for investigators using the rat for biomedical research.
Author List
Jacob HJ, Lazar J, Dwinell MR, Moreno C, Geurts AMAuthors
Melinda R. Dwinell PhD Associate Dean, Professor in the Physiology department at Medical College of WisconsinAron Geurts PhD Professor in the Physiology department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsEmbryo, Mammalian
Embryonic Stem Cells
Gene Knockout Techniques
Gene Targeting
Humans
Mice
Rats
