Kinetics of plasma membrane electron transport in a pulmonary endothelial cell-column. Ann Biomed Eng 1998;26(1):117-27
Date
06/04/1999Pubmed ID
10355556DOI
10.1114/1.83Scopus ID
2-s2.0-0031985797 (requires institutional sign-in at Scopus site) 5 CitationsAbstract
Thiazine dyes such as toluidine blue O (TBO) are reduced at the luminal endothelial surface. The purpose of this study was to determine the rate of this reaction in endothelial cells in culture. A multiple indicator dilution method was used to measure the reaction kinetics during transient passage of a TBO-containing bolus through a chromatographic column filled with bovine pulmonary arterial endothelial cells grown on microcarrier beads (cell-column). A bolus containing TBO and an inert extracellular reference indicator (FITC-Dextran) was injected upstream of the cell-column, and the indicator concentrations were measured downstream using on-line photodetection. The effects of column flow rate, PO2, and TBO concentration were studied. The fraction of TBO reduced upon passage through the cell-column decreased with increasing flow indicating that the reaction rate rather than TBO delivery controlled TBO reduction. The fraction of TBO reduced did not change with PO2 or dose in the ranges studied. TBO reduction was about 10 times that for steady state TBO sequestration by these cells which, along with the lack of a PO2 effect indicates that the rapid rate of reduction is not the rate-limiting step in steady state sequestration.
Author List
Olson LE, Merker MP, Bongard RD, Brantmeier BM, Audi SH, Linehan JH, Dawson CAAuthor
Said Audi PhD Professor in the Biomedical Engineering department at Marquette UniversityMESH terms used to index this publication - Major topics in bold
AnimalsCattle
Cell Membrane
Cell Membrane Permeability
Cells, Cultured
Chromatography
Coloring Agents
Dextrans
Electron Transport
Endothelium, Vascular
Fluorescein-5-isothiocyanate
Indicator Dilution Techniques
Linear Models
Optics and Photonics
Pulmonary Artery
Tolonium Chloride
