Interval mapping and congenic strains for a blood pressure QTL on rat chromosome 13. Mamm Genome 1997 Sep;8(9):636-41
Date
09/01/1997Pubmed ID
9271663DOI
10.1007/s003359900528Scopus ID
2-s2.0-0031227375 (requires institutional sign-in at Scopus site) 51 CitationsAbstract
The renin locus (Ren) on rat Chromosome (Chr) 13 had previously been shown to cosegregate with blood pressure in crosses involving Dahl salt-sensitive (S) and Dahl salt-resistant (R) rats. In the present work, interval mapping of blood pressure on Chr 13 with a large F2 (S x R), n = 233, population yielded a maximum LOD = 4.2 for linkage to blood pressure, but the quantitative trait locus (QTL) was only poorly localized to a large 35-centiMorgan (cM) segment of Chr 13. In the linkage analysis, the S-rat QTL allele (S) was associated with higher, and the R-rat QTL allele (R) with lower blood pressure, the difference between homozygotes being about 20 mm Hg. A congenic strain was made by introgressing the R-rat Ren allele into the recipient S strain. This congenic strain showed a 24 mm Hg reduction (P = 0.004) in blood pressure compared with S rats for rats fed 2% NaCl diet for 24 days; this difference was confirmed by two other independent tests. Two congenic substrains were derived from the first congenic strain with shorter R Chr 13 segments on the S background. Comparisons among these congenic strains showed that a blood pressure QTL was in the 24-cM chromosomal segment between Syt2 and D13M1Mit108. This segment does not include the renin locus, which is thus excluded from being the gene on rat Chr 13 responsible for genetic differences in blood pressure detected by linkage analysis.
Author List
Zhang QY, Dene H, Deng AY, Garrett MR, Jacob HJ, Rapp JPMESH terms used to index this publication - Major topics in bold
AnimalsBlood Pressure
Body Weight
Chromosome Mapping
Crosses, Genetic
Drug Resistance
Genetic Linkage
Genetic Markers
Genetics, Population
Haplotypes
Polymerase Chain Reaction
Polymorphism, Genetic
Rats
Rats, Inbred Strains
Renin
Sodium Chloride, Dietary
