Localization of the vasopressin V1a and V2 receptors within the renal cortical and medullary circulation. Am J Physiol 1997 Jul;273(1 Pt 2):R243-51
Date
07/11/1997Pubmed ID
9249556DOI
10.1152/ajpregu.1997.273.1.R243Scopus ID
2-s2.0-0031183039 (requires institutional sign-in at Scopus site) 77 CitationsAbstract
Arginine vasopressin (AVP) is a potent vasoconstrictor that preferentially reduces renal medullary blood flow through the stimulation of the vasopressin V1a receptor (V1aR). Studies have also shown that the vasopressin V2 receptor (V2R) may modulate AVP-mediated vasoconstriction. At present, the distribution of the V1aR and V2R within the renal cortical and medullary microcirculation has not been determined. This study was designed to localize the transcriptional and translational sites of the V1aR and V2R in microdissected intrarenal vascular segments from both the cortex and medulla, specifically the interlobar, arcuate, and interlobular arteries; afferent and efferent arterioles; glomeruli; and single outer medullary vasa recta capillaries using reverse transcription-polymerase chain reaction and Western blot analyses. The results indicated that V1aR mRNA and proteins were present in the isolated cortical or medullary vasculature, but the V2R mRNA and proteins were not found. This study suggests that the vasoconstrictor action of AVP within the renal medulla is mediated through the V1aR and that the modulatory V2R-mediated vasodilation is probably through the release of paracrine hormones found within the renal interstitial or tubular cells.
Author List
Park F, Mattson DL, Skelton MM, Cowley AW JrAuthor
Allen W. Cowley Jr PhD Professor in the Physiology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsArteries
Arterioles
Blotting, Western
DNA Primers
Kidney Cortex
Kidney Medulla
Male
Microcirculation
Muscle, Smooth, Vascular
Polymerase Chain Reaction
RNA, Messenger
Rats
Rats, Sprague-Dawley
Receptors, Vasopressin
Transcription, Genetic
