Tests of linkage and/or association of TGF-beta1 and COL1A1 genes with bone mass. Osteoporos Int 2005 Jan;16(1):86-92
Date
05/28/2004Pubmed ID
15164160DOI
10.1007/s00198-004-1650-1Scopus ID
2-s2.0-13244255482 (requires institutional sign-in at Scopus site) 14 CitationsAbstract
Transforming growth factor beta 1 (TGF-beta1) is involved in bone metabolism and collagen type I alpha 1 (COL1A1) is the most abundant protein of bone matrix. Both have been considered as candidate genes for osteoporosis. In this study, we employed the transmission disequilibrium test (TDT) to examine the relationship between each of the two genes with bone mineral density (BMD) and bone mineral content (BMC) at the spine and hip in a sample of 1668 subjects from 387 Caucasian nuclear families. For the TGF-beta1 gene, three SNPs, SNP1, SNP2, and SNP4 (located in exon 1, intron 4 and intron 5, respectively) were tested and the minor allele frequencies were 30.9%, 2.1% and 27.0%, respectively. All eight possible haplotypes (TGF1-8) were observed. For the COL1A1 gene, the minor allele frequencies of SNP5, SNP6 and SNP8 (located in exon 1, intron 1, and exon 45, respectively) were 15.2%, 18.7%, 2.0%, respectively, and only six of eight potential haplotypes (COL1-6) were obtained. In the whole sample, total associations were observed between haplotype COL5 with spine BMD (P=0.027), haplotypes COL3 and TGF4 with hip BMC (P=0.002, 0.003, respectively). Within-family associations were found for spine BMD at haplotypes TGF4 (P=0.027) in female offspring families and TGF3 (P=0.021) in male offspring families. Further studies with denser markers and larger sample size are required to eventually define the relationship between these two genes with bone mass at the spine and hip.
Author List
Long JR, Liu PY, Lu Y, Dvornyk V, Xiong DH, Zhao LJ, Deng HWMESH terms used to index this publication - Major topics in bold
AdultBone Density
Collagen Type I
Family Health
Female
Gene Frequency
Haplotypes
Hip
Humans
Linkage Disequilibrium
Male
Middle Aged
Parents
Phenotype
Polymorphism, Single Nucleotide
Spine
Transforming Growth Factor beta
Transforming Growth Factor beta1
