Extending the chinchilla middle ear epithelial model for mucin gene investigation. Int J Pediatr Otorhinolaryngol 2010 Sep;74(9):980-5
Date
07/02/2010Pubmed ID
20591507Pubmed Central ID
PMC2922454DOI
10.1016/j.ijporl.2010.05.009Scopus ID
2-s2.0-77955557365 (requires institutional sign-in at Scopus site) 10 CitationsAbstract
OBJECTIVES: To investigate the expression of recently identified human mucin genes in an in vivo model of the chinchilla middle ear epithelium (CMEE).
METHODS: CMEE was harvested, RNA was extracted and primers were designed for RT-PCR to assess for expression of mucin genes Muc6, Muc17 and Muc18. Further sequencing of these genes was also accomplished.
RESULTS: Mucin genes Muc6, Muc17 and Muc18 was assessed and found to be identical to the expression in human and mouse MEE.
CONCLUSION: This study further characterizes mucin gene expression in the CMEE and provides additional sequence data for chinchilla middle ear genes. The concordance of this gene expression data to that of both the human and mouse models further demonstrates the utility of this animal model in OM investigations.
Author List
Kerschner JE, Khampang P, Samuels TMESH terms used to index this publication - Major topics in bold
AnimalsChinchilla
Ear, Middle
Epithelium
Gastric Mucosa
Humans
Intestine, Small
Mice
Mucin-6
Mucins
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
