Basic calcium phosphate crystals induce synthesis and secretion of 92 kDa gelatinase (gelatinase B/matrix metalloprotease 9) in human fibroblasts. Ann Rheum Dis 1998 Jan;57(1):56-60
Date
04/16/1998Pubmed ID
9536826Pubmed Central ID
PMC1752460DOI
10.1136/ard.57.1.56Scopus ID
2-s2.0-0031900306 (requires institutional sign-in at Scopus site) 18 CitationsAbstract
OBJECTIVE: Synovial fluid basic calcium phosphate (BCP) crystals are associated with severe joint degeneration accompanied by synovial hypertrophy. The metalloprotease 92 kDa gelatinase (MMP-9) has been implicated in the degradation of extracellular matrix in osteoarthritis, but the ability of BCP crystals to induce gelatinase in human fibroblasts or in adult porcine chondrocytes has not previously been studied. The hypothesis that the mitogenic response to BCP crystals is accompanied by induction and secretion of MMP-9 was studied.
METHODS: MMP-9 messenger RNA (mRNA) was detected by northern blot and reverse transcription-polymerase chain reaction (RT-PCR). Gelatinase secretion was identified by western blot and zymography of conditioned media.
RESULTS: BCP crystals caused a concentration dependent induction of MMP-9 mRNA accumulation and protein secretion in human fibroblasts but not in adult porcine chondrocytes.
CONCLUSION: BCP crystals induce MMP-9 production by HF but not adult porcine chondrocytes. Fibroblast MMP-9 may be an important mediator of the joint destruction associated with synovial fluid BCP crystals.
Author List
McCarthy GM, Macius AM, Christopherson PA, Ryan LM, Pourmotabbed TMESH terms used to index this publication - Major topics in bold
AnimalsBlotting, Northern
Calcium Phosphates
Cells, Cultured
Chondrocytes
Collagenases
Crystallization
Dose-Response Relationship, Drug
Electrophoresis, Polyacrylamide Gel
Fibroblasts
Humans
Immunoblotting
Matrix Metalloproteinase 9
Polymerase Chain Reaction
RNA, Messenger
Stimulation, Chemical
Swine
