Faculty Collaboration Database

Anti-interleukin (IL)-4 and -IL-5 antibodies downregulate IgE and eosinophilia in mice exposed to Aspergillus antigens. Allergy 1997 Dec;52(12):1215-21

Date

02/05/1998

Pubmed ID

9450141

DOI

10.1111/j.1398-9995.1997.tb02526.x

Scopus ID

2-s2.0-0031456652 (requires institutional sign-in at Scopus site)   49 Citations

Abstract

The effect of multiple divided doses compared with single-dose injections of antibodies to murine interleukin (IL)-4 and IL-5 in their respective downregulation of IgE and eosinophilia developing in a model of allergic aspergillosis is investigated. BALB/c mice were exposed to Aspergillus fumigatus antigens (Af) before and along with anticytokine antibodies. The kinetics of blood eosinophils, eosinophil peroxidase (EPO) in bone-marrow cells, serum levels of IgE and Af-specific antibodies, Af-induced cytokine production and mRNA, and lung histology were studied. The results indicate that only multiple anti-IL-5 antibodies were effective in maintaining baseline levels of blood eosinophils. Multiple anti-IL-4 antibodies also downregulated eosinophils in the bone marrow, lung, and peripheral blood, although to a lesser extent than in anti-IL-5 antibody-injected mice. Significant correlation between the EPO activity and the eosinophil numbers in anticytokine antibody-treated mice was observed. The different anti-IL-4 antibody treatments downregulated IgE to the same extent. We conclude that multiple divided doses of anti-IL-5 antibodies are required to sustain normal eosinophil levels in murine allergic aspergillosis. This information may be significant in the therapy of pulmonary allergic diseases.

Author List

Kurup VP, Murali PS, Guo J, Choi H, Banerjee B, Fink JN, Coffman RL

MESH terms used to index this publication - Major topics in bold

Animals
Antibodies, Fungal
Antibodies, Monoclonal
Aspergillosis, Allergic Bronchopulmonary
Aspergillus fumigatus
Bone Marrow
Down-Regulation
Eosinophil Peroxidase
Eosinophilia
Eosinophils
Immunoglobulin E
Immunoglobulin G
Interleukin-4
Interleukin-5
Leukocyte Count
Lung
Mice
Mice, Inbred BALB C
Peroxidases
RNA, Messenger
Spleen